ABSTRACT
Objective:To analyze the chemical constituents of the volatile constituents of Flos chrysanthemi indici.Methods:The volatile constituents in Flos chrysanthemi indici was extracted by the method of Solid phase microextraction (SPME). The components of the volatile constituents were separated and identified by gas chromatography-mass spectrometry (GC-MS). The relative content of each component was determined by area normalization.Results:Fifty different compounds were isolated and identified, accounting for 97.17% of the total volatile constituents. The main components in volatile constituents of Flos chrysanthemi indici were eucalyptol (24.72%), á-Myrcene (15.78%), 1,6,10-Dodecatriene, 7,11-dimethyl-3-methylene-, (Z)- (10.44%), Camphor (10.05%), Naphthalene, 1,2,3,4,4a,5,6,8a-octahydro-7-methyl-4-methylene-1-(1-methylethyl)-, (1à,4aà,8aà)- (4.81%), á-Phellandrene (4.43%), 1R-à-Pinene (4.28%), Bornyl acetate (2.56%), Bicyclo [3.1.0] hexane, 4-methylene-1-(1-methylethyl)- (1.59%), 1H-Benzocycloheptene, 2,4a,5,6,7,8-hexahydro-3,5,5,9-tetramethyl-, (R)-(1.47%). Conclusions:The SPME technology can be used for volatile component analysis of wild chysanthemum,and it can help to improve the sensitivity and accuracy.
ABSTRACT
Objective@#To analyze the chemical constituents of the volatile constituents of Flos chrysanthemi indici.@*Methods@#The volatile constituents in Flos chrysanthemi indici was extracted by the method of Solid phase microextraction (SPME). The components of the volatile constituents were separated and identified by gas chromatography-mass spectrometry (GC-MS). The relative content of each component was determined by area normalization.@*Results@#Fifty different compounds were isolated and identified, accounting for 97.17% of the total volatile constituents. The main components in volatile constituents of Flos chrysanthemi indici were eucalyptol (24.72%), á-Myrcene (15.78%), 1,6,10-Dodecatriene, 7,11-dimethyl-3-methylene-, (Z)- (10.44%), Camphor (10.05%), Naphthalene, 1,2,3,4,4a,5,6,8a-octahydro-7-methyl-4-methylene-1-(1-methylethyl)-, (1à,4aà,8aà)- (4.81%), á-Phellandrene (4.43%), 1R-à-Pinene (4.28%), Bornyl acetate (2.56%), Bicyclo [3.1.0] hexane, 4-methylene-1-(1-methylethyl)- (1.59%), 1H-Benzocycloheptene, 2,4a,5,6,7,8-hexahydro-3,5,5,9-tetramethyl-, (R)-(1.47%).@*Conclusions@#The SPME technology can be used for volatile component analysis of wild chysanthemum,and it can help to improve the sensitivity and accuracy.
ABSTRACT
A new method for the rapid determination of total phthalates (PAEs) in edible oils was developed. The PAEs in edible oils all were hydrolyzed to phthalic acid with tetrabutylammonium chloride (TBAC) as catalyst. Then phthalic acid was extracted by the supramolecular solvent ( SUPRAS) made up of octanol, tetrahydrofuran and aqueous solution, and detected by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS). As a result, hydrolysis time was 10 min. The linear range of phthalic acid was 0. 05- 2. 0 mg / L with a good correlation coefficients ( r > 0. 999). The limits of detection ( LOD) and quantification (LOQ) were 5. 41 and 18. 05 μg / kg, respectively. The recoveries of target analyte at three spiked levels were in the range of 84. 6% - 104. 5% . The repeatability, expressed as relative standard deviation (RSD), was 2. 6% for intra-day and 3. 7% for inter-day. The total PAEs content of 12 edible oils was found in the range of 0. 30-1. 09 mg / kg.
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Objective To explore the relationship between blood pressure changes and prognosis in patients with acute stroke by ambulatory blood pressure monitoring. Methods 79 patients with acute stroke were divided into two groups: cerebral infarction group (n=42) and cerebral hemorrhage group (n=37). Blood pressure of patients in both groups was monitored, and the score of handicap was evaluated respectively on 30d after onset. Results (1)Blood pressure was elevated in 87.3% of the patients with stroke at acute stage and then lowered spontaneously. Compared with that of the 1st day after onset, blood pressure lowered significantly from [SBP (155.9?21.7), DBP (96.6?20.8) mmHg] to [SBP (147.6?17.2), DBP (85.6?13.6) mmHg] (P